Unit-1
Introduction to Computers and Biological Databases
Computer Basics :
Basic Computer Operations: I/O Units; Computer
Memory; Processor; Binary Arithmetic; Logic
Circuit; Architecture; Operating Systems and
application softwares.
Biological Databases :
Classification of Databases – Raw and Processed
Databases; Primary (NCBI), Secondary (PIR) and
Tertiary or Composite (KEGG) Databases; Structure
and Sequence Databases.
Specialized Databases – Protein Pattern Databases;
Protein Structure and Classification Databases
(CATH/SCOP). Genome Information Resources:
DNA Sequence Databases Specialized Genomic
Resources.
Protein Databases based on Composition, Motifs
and Patterns.
Protein Structure Visualization Software.
Unit-2
BLAST and sequence alignment
BLAST and Sequence Alignment: BLAST and its Types; Retrieving Sequence using BLAST.
Pairwise Alignment: Identity and Similarity; Global and Local Alignment; Pairwise Database Searching. Multiple Sequence Alignment: Goal of Multiple Sequence Alignment; Computational Complexity; Manual Methods; Simultaneous Methods; Progressive Methods; Databases of Multiple Alignment; Secondary Database Searching; Analysis Packages; MSA.
